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1

Use of Triazine dyes in the affinity chromatographic purification of alkaline phosphatase from calf intestine

Visilis Bouriotis, Peter D.G. Dean

Journal:

Journal of Chromatography A

Year:

1981

Language:

english

File:

PDF, 651 KB

english, 1981

2

High-performance liquid chromatography for the purification of restriction endonucleases, application to BanII, SacI, and SphI

V. Bouriotis, A. Zafeiropoulos, Y.D. Clonis

Journal:

Analytical Biochemistry

Year:

1987

Language:

english

File:

PDF, 657 KB

english, 1987

3

Chitin deacetylation by enzymatic means: monitoring of deacetylation processes

A. Martinou, D. Kafetzopoulos, V. Bouriotis

Journal:

Carbohydrate Research

Year:

1995

Language:

english

File:

PDF, 417 KB

english, 1995

4

Isolation of chitin deacetylase from Mucor rouxii by immunoaffinity chromatography

A. Martinou, D. Kafetzopoulos, V. Bouriotis

Journal:

Journal of Chromatography A

Year:

1993

Language:

english

File:

PDF, 411 KB

english, 1993

5

Sequence-specific DNA affinity chromatography: Application of a group-specific adsorbent for the isolation of restriction endonucleases

C. Pozidis, G. Vlatakis, V. Bouriotis

Journal:

Journal of Chromatography A

Year:

1993

Language:

english

File:

PDF, 325 KB

english, 1993

6

Carbohydrate esterase family 4 enzymes: substrate specificity

Frederic Caufrier, Aggeliki Martinou, Claude Dupont, Vassilis Bouriotis

Journal:

Carbohydrate Research

Year:

2003

Language:

english

File:

PDF, 410 KB

english, 2003

7

Mode of action of chitin deacetylase from Mucor rouxii on partially N-acetylated chitosans

Aggeliki Martinou, Vassilis Bouriotis, Bjørn T. Stokke, Kjell M. Vårum

Journal:

Carbohydrate Research

Year:

1998

Language:

english

File:

PDF, 203 KB

english, 1998

8

Applications of immobilised phenylboronic acids as supports for group-specific ligands in the affinity chromatography of enzymes

Vasilis Bouriotis, Ian J. Galpin, P.D.G. Dean

Journal:

Journal of Chromatography A

Year:

1981

Language:

english

File:

PDF, 786 KB

english, 1981

9

Affinity partitioning of restriction endonucleases : Application to the purification of EcoR I and EcoR V

G. Vlatakis, V. Bouriotis

Journal:

Journal of Chromatography A

Year:

1991

Language:

english

File:

PDF, 670 KB

english, 1991

10

Cloning and expression of a chitin deacetylase gene (CDA2) from Saccharomyces cerevisiae in Escherichia coli: Purification and characterization of the cobalt-dependent recombinant enzyme

Aggeliki Martinou, Dimitris Koutsioulis, Vassilis Bouriotis

Journal:

Enzyme and Microbial Technology

Year:

2003

Language:

english

File:

PDF, 293 KB

english, 2003

11

Stereospecificity of hydrogen transfer by the NAD+-linked alcohol dehydrogenase from the Antarctic psychrophile Moraxella sp. TAE123

Kelly Velonia, Iason Tsigos, Vassilis Bouriotis, Ioulia Smonou

Journal:

Bioorganic & Medicinal Chemistry Letters

Year:

1999

Language:

english

File:

PDF, 180 KB

english, 1999

12

Engineering the properties of a cold active enzyme through rational redesign of the active site

Iason Tsigos, Konstantinos Mavromatis, Maria Tzanodaskalaki, Charalambos Pozidis, Michael Kokkinidis, Vassilis Bouriotis

Journal:

Year:

2001

Language:

english

File:

PDF, 470 KB

english, 2001

13

Exploring the role of a glycine cluster in cold adaptation of an alkaline phosphatase

Konstantinos Mavromatis, Iason Tsigos, Maria Tzanodaskalaki, Michael Kokkinidis, Vassilis Bouriotis

Journal:

Year:

2002

Language:

english

File:

PDF, 475 KB

english, 2002

14

Alkaline phosphatase from the Antarctic strain TAB5 : Properties and psychrophilic adaptations

Maria Rina, Charalambos Pozidis, Konstantinos Mavromatis, Maria Tzanodaskalaki, Michael Kokkinidis, Vassilis Bouriotis

Journal:

Year:

2000

Language:

english

File:

PDF, 1.39 MB

english, 2000

15

Coordination sphere of the third metal site is essential to the activity and metal selectivity of alkaline phosphatases

Dimitris Koutsioulis, Andrzej Lyskowski, Seija Mäki, Ellen Guthrie, Georges Feller, Vassilis Bouriotis, Pirkko Heikinheimo

Journal:

Protein Science

Year:

2010

Language:

english

File:

PDF, 284 KB

english, 2010

16

Mode of action and antifungal properties of two cold-adapted chitinases

Konstantinos Mavromatis, Matteo Lorito, SheridanL. Woo, Vassilis Bouriotis

Journal:

Year:

2003

Language:

english

File:

PDF, 244 KB

english, 2003

17

Sequence-specific DNA affinity chromatography: Application to the purification of EcoRI and SphI

G. Vlatakis, V. Bouriotis

Journal:

Analytical Biochemistry

Year:

1991

Language:

english

File:

PDF, 957 KB

english, 1991

18

Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus : Functional insights from structural data

Vasiliki E. Fadouloglou, Alexandra Deli, Nicholas M. Glykos, Emmanuel Psylinakis, Vassilis Bouriotis, Michael Kokkinidis

Journal:

Year:

2007

Language:

english

File:

PDF, 1.15 MB

english, 2007

19

Isolation and characterization of CspBI, a novel NotI isoschizomer from Corynebacterium species B recognizing 5′-GC↓GGCCGC-3′

Nikolai N. Sokolov, Michael A. Eldarov, Mary Rina, Sergey V. Korolev, Maria Markaki, Alexei A. Kalugin, Natalia M. Omelyanuk, Konstantin G. Skryabin, Vassilis Bouriotis

Journal:

Year:

1998

Language:

english

File:

PDF, 474 KB

english, 1998

20

Dye-ligand chromatography for the resolution and purification of restriction endonucleases

G. Vlatakis, G. Skarpelis, I. Stratidaki, V. Bouriotis, Y. D. Clonis

Journal:

Applied Biochemistry and Biotechnology

Year:

1987

Language:

english

File:

PDF, 912 KB

english, 1987

21

Enzyme activity determination on macromolecular substrates by isothermal titration calorimetry: application to mesophilic and psychrophilic chitinases

Thierry Lonhienne, Etienne Baise, Georges Feller, Vassilis Bouriotis, Charles Gerday

Journal:

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology

Year:

2001

Language:

english

File:

PDF, 238 KB

english, 2001

22

Chitin deacetylases: new, versatile tools in biotechnology

Iason Tsigos, Aggeliki Martinou, Dimitris Kafetzopoulos, Vassilis Bouriotis

Journal:

Trends in Biotechnology

Year:

2000

Language:

english

File:

PDF, 136 KB

english, 2000

23

Purification and characterization of an alcohol dehydrogenase from the Antarctic psychrophile Moraxella sp. TAE123

Iason Tsigos, Kelly Velonia, Ioulia Smonou, Vassilis Bouriotis

Journal:

Year:

1998

Language:

english

File:

PDF, 167 KB

english, 1998

24

Mode of action of chitin deacetylase from Mucor rouxii on N-acetylchitooligosaccharides

Iason Tsigos, Nathalie Zydowicz, Aggeliki Martinou, Alain Domard, Vassilis Bouriotis

Journal:

Year:

1999

Language:

english

File:

PDF, 578 KB

english, 1999

25

Modular structure, local flexibility and cold-activity of a novel chitobiase from a psychrophilic antarctic bacterium

Thierry Lonhienne, Jérôme Zoidakis, Constantinos E Vorgias, Georges Feller, Charles Gerday, Vassilis Bouriotis

Journal:

Journal of Molecular Biology

Year:

2001

Language:

english

File:

PDF, 161 KB

english, 2001

26

Expression, Purification, and Characterization of a Cobalt-Activated Chitin Deacetylase (Cda2p) from Saccharomyces cerevisiae

Aggeliki Martinou, Dimitris Koutsioulis, Vassilis Bouriotis

Journal:

Protein Expression and Purification

Year:

2002

Language:

english

File:

PDF, 106 KB

english, 2002

27

Sequence of the cloned bseCIM gene: M · BseCI reveals high homology to M · BanIII

Maria Rina, Maria Markaki, Vassilis Bouriotis

Journal:

Year:

1994

Language:

english

File:

PDF, 315 KB

english, 1994

28

Crystal Structure of Alkaline Phosphatase from the Antarctic Bacterium TAB5

Ellen Wang, Dimitris Koutsioulis, Hanna-Kirsti S. Leiros, Ole Andreas Andersen, Vassilis Bouriotis, Edward Hough, Pirkko Heikinheimo

Journal:

Journal of Molecular Biology

Year:

2007

Language:

english

File:

PDF, 1.90 MB

english, 2007

29

Yeast ascospore wall assembly requires two chitin deacetylase isozymes

Anna Christodoulidou, Peter Briza, Adi Ellinger, Vassilis Bouriotis

Journal:

Year:

1999

Language:

english

File:

PDF, 210 KB

english, 1999

30

Cloning and characterization of the gene encoding PspPI methyltransferase from the Antarctic psychrotroph Psychrobacter sp. strain TA137.: Predicted interactions with DNA and organization of the variable region

Maria Rina, Frederic Caufrier, Maria Markaki, Konstantinos Mavromatis, Michael Kokkinidis, Vassilis Bouriotis

Journal:

Year:

1997

Language:

english

File:

PDF, 477 KB

english, 1997

31

Distinct functions of polysaccharide deacetylases in cell shape, neutral polysaccharide synthesis and virulence of Bacillus anthracis

Balomenou, Stavroula, Fouet, Agnes, Tzanodaskalaki, Mary, Couture-Tosi, Evelyne, Bouriotis, Vassilis, Boneca, Ivo G.

Journal:

Molecular Microbiology

Year:

2013

Language:

english

File:

PDF, 1.31 MB

english, 2013

32

A critical role for peptidoglycan N-deacetylation in Listeria evasion from the host innate immune system

Boneca, Ivo G., Dussurget, Olivier, Cabanes, Didier, Nahori, Marie-Anne, Sousa, Sandra, Lecuit, Marc, Psylinakis, Emmanuel, Bouriotis, Vassilis, Hugot, Jean-Pierre, Giovannini, Marco, Coyle, Anthony,

Journal:

Proceedings of the National Academy of Sciences

Year:

2007

Language:

english

File:

PDF, 916 KB

english, 2007

33

Cloning, purification and characterization of the BseCI DNA methyltransferase from Bacillus stearothermophilus

Rina, M., Bouriotis, V.

Journal:

Year:

1993

Language:

english

File:

PDF, 586 KB

english, 1993

34

Isolation and partial characterization of two alcohol dehydrogenase isozymes from the medfly Ceratitis capitata

Gasperi, G., Kafetzopoulos, D., Christodoulidou, A., Bouriotis, V., Savakis, C.

Journal:

Insect Biochemistry and Molecular Biology

Year:

1994

Language:

english

File:

PDF, 1.37 MB

english, 1994

35

Impact of Protein Flexibility on Hydride-Transfer Parameters in Thermophilic and Psychrophilic Alcohol Dehydrogenases

Liang, Zhao-Xun, Tsigos, Iason, Bouriotis, Vassilis, Klinman, Judith P.

Journal:

Journal of the American Chemical Society

Year:

2004

Language:

english

File:

PDF, 90 KB

english, 2004

36

Molecular Dynamics Simulations of BcZBP , A Deacetylase from Bacillus cereus : Active Site Loops Determine Substrate Accessibility and Specificity

Fadouloglou, Vasiliki E., Stavrakoudis, Athanassios, Bouriotis, Vassilis, Kokkinidis, Michael, Glykos, Nicholas M.

Journal:

Journal of Chemical Theory and Computation

Year:

2009

Language:

english

File:

PDF, 7.29 MB

english, 2009

37

Impact of Protein Flexibility on Hydride-Transfer Parameters in Thermophilic and Psychrophilic Alcohol Dehydrogenases [ J. Am. Chem. Soc . 2004 , 126 , 9500−9501].

Liang, Zhao-Xun, Tsigos, Iason, Bouriotis, Vassilis, Klinman, Judith P.

Journal:

Journal of the American Chemical Society

Year:

2006

Language:

english

File:

PDF, 9 KB

english, 2006

38

Evidence for Increased Local Flexibility in Psychrophilic Alcohol Dehydrogenase Relative to Its Thermophilic Homologue †

Liang, Zhao-Xun, Tsigos, Iason, Lee, Thomas, Bouriotis, Vassilis, Resing, Katheryn A., Ahn, Natalie G., Klinman, Judith P.

Journal:

Year:

2004

Language:

english

File:

PDF, 259 KB

english, 2004

39

Purification and Characterization of Chitin Deacetylase from Colletotrichum lindemuthianum

Tsigos, I., Bouriotis, V.

Journal:

Journal of Biological Chemistry

Year:

1995

Language:

english

File:

PDF, 616 KB

english, 1995

40

N -Acetylglucosamine Deacetylases Modulate the Anchoring of the Gamma-Glutamyl Capsule to the Cell Wall of Bacillus anthracis

Candela, Thomas, Balomenou, Stavroula, Aucher, Willy, Bouriotis, Vassilis, Simore, Jean-Pierre, Fouet, Agnes, Boneca, Ivo G.

Journal:

Microbial Drug Resistance

Year:

2014

Language:

english

File:

PDF, 862 KB

english, 2014

41

The Primary Structure of a Fungal Chitin Deacetylase Reveals the Function for Two Bacterial Gene Products

Dimitris Kafetzopoulos, George Thireos, John N. Vournakis and Vassilis Bouriotis

Journal:

Proceedings of the National Academy of Sciences

Year:

1993

Language:

english

File:

PDF, 656 KB

english, 1993

42

Two Sporulation-specific Chitin Deacetylase-encoding Genes Are Required for the Ascospore Wall Rigidity of Saccharomyces cerevisiae

Christodoulidou, A., Bouriotis, V., Thireos, G.

Journal:

Journal of Biological Chemistry

Year:

1996

Language:

english

File:

PDF, 922 KB

english, 1996

43

Peptidoglycan N-Acetylglucosamine Deacetylases from Bacillus cereus, Highly Conserved Proteins in Bacillus anthracis

Psylinakis, E., Boneca, I. G., Mavromatis, K., Deli, A., Hayhurst, E., Foster, S. J., Varum, K. M., Bouriotis, V.

Journal:

Journal of Biological Chemistry

Year:

2005

Language:

english

File:

PDF, 923 KB

english, 2005

44

Isolation and identification of restriction endonuclease BseCI

Rina, M., Dialektakis, D., Clark, D., Pagomenou, M., Bouriotis, V.

Journal:

Nucleic Acids Research

Year:

1992

Language:

english

File:

PDF, 1.07 MB

english, 1992

45

Isolation and identification of restriction endonuclease BshFI

Vlatakis, George, Clark, Duncan, Bouriotis, Vassilis

Journal:

Nucleic Acids Research

Year:

1989

Language:

english

File:

PDF, 862 KB

english, 1989

46

Isolation and identification of restriction endonuclease BsiSI

Rina, Mary, Bouriotis, Vassilis

Journal:

Nucleic Acids Research

Year:

1990

Language:

english

File:

PDF, 1018 KB

english, 1990

47

Structure determination through homology modelling and torsion-angle simulated annealing: application to a polysaccharide deacetylase from Bacillus cereus

Fadouloglou, Vasiliki E., Kapanidou, Maria, Agiomirgianaki, Athanasia, Arnaouteli, Sofia, Bouriotis, Vassilis, Glykos, Nicholas M., Kokkinidis, Michael

Journal:

Acta Crystallographica Section D Biological Crystallography

Year:

2013

Language:

english

File:

PDF, 2.39 MB

english, 2013

48

Structure determination of BA0150, a putative polysaccharide deacetylase from Bacillus anthracis

Strunk, Robert J., Piemonte, Katrina M., Petersen, Natasha M., Koutsioulis, Dimitris, Bouriotis, Vassilis, Perry, Kay, Cole, Kathryn E.

Journal:

Acta Crystallographica Section F Structural Biology Communications

Year:

2014

Language:

english

File:

PDF, 458 KB

english, 2014

49

Cloning, Sequences, and Characterization of Two Chitinase Genes from the Antarctic Arthrobacter sp. Strain TAD20: Isolation and Partial Characterization of the Enzymes

Lonhienne, T., Mavromatis, K., Vorgias, C. E., Buchon, L., Gerday, C., Bouriotis, V.

Journal:

Journal of Bacteriology

Year:

2001

Language:

english

File:

PDF, 412 KB

english, 2001

50

Isolation and identification of restriction endonuclease BshGI

Scarpelis, George, Moissidou, Athina, Rina, Mary, Bouriotis, Vassilis

Journal:

Nucleic Acids Research

Year:

1989

Language:

english

File:

PDF, 877 KB

english, 1989